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Retracted : microRNA‐145 regulates tumor suppressor candidate 3 and mitogen‐activated protein kinase pathway to inhibit the progression of colorectal cancer
Author(s) -
Tang Hanqing,
Li Keming,
Zheng Jianyu,
Dou Xibin,
Zhao Yufeng,
Wang Luyao
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28122
Subject(s) - viability assay , mapk/erk pathway , cancer research , microrna , signal transduction , kinase , western blot , biology , gene knockdown , microbiology and biotechnology , cell , apoptosis , biochemistry , gene
Background It has been reported that microRNA‐145 (miR‐145) is downregulated in various cancers, including colorectal cancer (CRC). However, the role of miR‐145 in progress of CRC and its mechanism remains unclear. Methods The expressions of miR‐145 and tumor suppressor candidate 3 (TUSC3) were determined in CRC tissues and cells by real‐time quantitative polymerase chain reaction and Western blot analysis. The effects of miR‐145 and TUSC3 on cell viability, migration, and invasion of CRC cells were examined by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐trtrazolium bromide assay and trans‐well chamber experiment, respectively. The interaction between miR‐145 and TUSC3 was explored by bioinformatics analysis, luciferase reporter assay, and Western blot analysis. The abundances of mitogen‐activated protein kinase (MAPK) signaling pathway‐related proteins were measured by Western blot analysis. Results miR‐145 expression was downregulated in CRC tissues and cell lines, and TUSC3 was upregulated in CRC tissues and correlated inversely with miR‐145 abundance. Overexpression of miR‐145 and knockdown of TUSC3 suppressed cell viability, migration, and invasion in LS174T and HCT116 cells. Moreover, TUSC3 was indicated as a novel target of miR‐145 and its expression was negatively regulated by miR‐145. Restoration of TUSC3 can partially reverse the inhibitory effects of miR‐145 on phosphorylation of extracellular signal‐regulated kinases 1 and 2 in CRC cells. Conclusion miR‐145 can inhibit the viability, migration, and invasion through addressing MAPK signaling pathway by targeting TUSC3 in CRC cells, providing a novel biomarker for treatment of CRC.