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The interaction between ANXA2 and lncRNA Fendrr promotes cell apoptosis in caerulein‐induced acute pancreatitis
Author(s) -
Zhao Dali,
Ge Huajun,
Ma Biao,
Xue Dongbo,
Zhang Weihui,
Li Zhituo,
Sun Haijun
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28097
Subject(s) - propidium iodide , annexin a2 , apoptosis , annexin , flow cytometry , microbiology and biotechnology , western blot , cancer research , chemistry , biology , programmed cell death , biochemistry , gene
Abstract Background Annexin A2 (ANXA2) plays a crucial role in acute pancreatitis (AP). However, its potential mechanism remains unclear. Methods In the present study, we used caerulein‐treated AR42J rat pancreatic acinar cells as cell model of AP to investigate the potential functions of ANXA2 and its predicted long noncoding RNA (lncRNA) FOXF1 adjacent noncoding developmental regulatory RNA (lncRNA Fendrr). Cell apoptosis was evaluated by flow cytometry using annexinV‐fluorescein isothiocyanate/propidium iodide staining. The expressions of ANAX2 and lncRNA Fendrr were detected by quantitative real‐time polymerase chain reaction (qRT‐PCR). Furthermore, Western blot analysis was performed to determine the protein levels of ANXA2, Bcl‐2, and Bax. The association between lncRNA Fendrr and ANXA2 was disclosed by RNA pull‐down, RNA immunoprecipitation, and electrophoretic mobility shift assays. Results ANXA2 was elevated in caerulein‐induced AP model and promoted apoptosis of AR42J cells. LncRNA Fendrr was also upregulated in AP cell model and directly bound ANXA2 protein. Further studies indicated that the interaction between ANXA2 and lncRNA Fendrr contributed to the apoptosis of AR42J cells in AP cell model. Conclusion Our study demonstrated that ANXA2 promoted AP progression via interacting with lncRNA Fendrr in vitro, which will provide a novel insight into the therapeutic target for AP.