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Long noncoding RNA uc.4 inhibits cell differentiation in heart development by altering DNA methylation
Author(s) -
Zhang Qijun,
Feng Mengwen,
Zhang Hao,
Xu Jia,
Zhang Li,
Wang Xuejun,
Cheng Zijie,
Qian Lingmei
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.28084
Subject(s) - biology , dna methylation , kegg , zebrafish , differentially methylated regions , gene , methylated dna immunoprecipitation , methylation , microbiology and biotechnology , genetics , gene expression , transcriptome
In previous studies, we have demonstrated that long noncoding RNA uc.4 may influence the cell differentiation through the TGF‐β signaling pathway, suppressed the heart development of zebrafish and resulting cardiac malformation. DNA methylation plays a significant role in the heart development and disordered of DNA methylation may cause disruption of control of gene promoter. In this study, methylated DNA immunoprecipitation was performed to identify the different expression levels of methylation regions. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were also performed to identify the possible biological process and pathway that uc.4 may join, associated with Rap1 signaling pathway, gonadotropin‐releasing hormone signaling pathway, and Calcium signaling pathway. We found that the distribution of differentially methylated regions peaks was mainly located in intergenic and intron regions. Altogether, our result showed that differentially methylated genes are significantly expressed in uc.4‐overexpression cells, providing valuable data for further exploration of the role of uc.4 in heart development.