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Effect of single‐nucleotide polymorphism in pri‐microRNA‐124 on poststroke motor function recovery
Author(s) -
Chen Shangjun,
Chen Yuhong,
Gao Yadong,
Zuo Yi,
Zhou Xiaowei
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27986
Subject(s) - single nucleotide polymorphism , motor function , motor dysfunction , western blot , medicine , allele , downregulation and upregulation , microrna , polymorphism (computer science) , stroke (engine) , motor system , biology , bioinformatics , genotype , physical medicine and rehabilitation , endocrinology , oncology , gene , neuroscience , genetics , disease , mechanical engineering , engineering
Background Stroke is a leading cause of long‐term disability and further one of the main causes of motor impairment. The current study aimed to investigate whether miR‐124 polymorphism (rs531564) influences the motor function of patients after stroke. Methods In total, 56 patients with stroke‐induced motor dysfunction were enrolled. Box and block test (BBT) and Fugl‐Meyer assessment (FMA) were performed to evaluate the motor functions in all participants. Computation analysis, luciferase activity, PCR assays, and Western blot analysis were performed to reveal the molecular mechanism underlying the effect of miR‐124 polymorphism on motor functions. Results The 56 participants were genotyped as CC, CG, and GG, respectively. The serum miR‐124 was significantly upregulated in the CC group than that in the GC and GG groups. According to the result of FMA or BBT, there was no obvious difference in upper and lower limb motor functions between CC and CG/GG groups 1 week after the treatment. In addition, scores of BBT and FMA exhibited apparent improvement in both groups at 1 month and 3 months after the treatment. Furthermore, improvements in the CG/GG groups were more significant as compared with those in the CC group. CDK4 was a target of miR‐124, and the effect of miR‐124 on the motor function recovery might be mediated by CDK4. Conclusion The presence of a minor allele, G, of miR‐124 polymorphism (rs531564) reduced the expression of miRNA and upregulated the expression of CDK4, which may contribute to the effect of rs531564 on the motor function recovery in poststroke patients.