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The expression of interleukin‐15 and interleukin‐17 in tears and orbital tissues of Graves ophthalmopathy patients
Author(s) -
Chen Qun
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27916
Subject(s) - tears , interleukin 1β , interleukin , interleukin 15 , expression (computer science) , medicine , interleukin 20 , interleukin 5 , immunology , cytokine , computer science , programming language
Abstract Objective The objective of this study was to study the levels of interleukin (IL)‐15 and IL‐17 in tears and orbital tissues of the patients with the Graves ophthalmopathy (GO) (active and inactive stages) and in controls. Methods Twenty‐four active GO patients (CAS ≥ 3/7) were collected in our research. All patients were treated with corticosteroids during the therapy process (4‐15 months) and developed into the inactive phase (CAS ≤ 2/7). The tear samples were gathered from inactive and active stages of patients with GO and sex‐matched and age‐matched controls (volunteers, n = 24) for the enzyme‐linked immunosorbent assay measurement of IL‐15 and IL‐17. The orbital tissues were collected from inactive stage of patients with GO and healthy volunteers for the analysis of IL‐15 and IL‐17 positive cells using the immunohistochemical analysis. Results In comparison with the volunteers, significant upregulation of IL‐15 and IL‐17 were identified in tears of active ( P < 0.05) and inactive patients with GO ( P < 0.01). Compared with inactive patients with GO, the levels of IL‐15 and IL‐17 in the tears were obviously higher than those in the active patients with GO. In addition, higher expression of IL‐15 and IL‐17 positive cells were found in the orbital organization of inactive patients with GO compared with volunteers. Conclusion The differences in IL‐15 and IL‐17 expression between GO patients (active and inactive stages) and controls suggested both IL‐15 and IL‐17 expression were significantly correlated with thyroid‐associated ophthalmopathy pathogenesis and development.