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Association of TNF‐308 G>A polymorphism located in tumor necrosis factor a with the risk of developing cervical cancer and results of pap smear
Author(s) -
Babapour Nooshin,
Mehramiz Mehrane,
Rastgar Moghadam Azam,
Behboodi Negin,
Yousefi Zohre,
Maftouh Mona,
Talebian Sahar,
Khazaei Majid,
Jafarian Amirhosein,
SharifiSistani Noorieh,
Avan Amir,
Hasanzadeh Malihe
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27823
Subject(s) - cervical cancer , minor allele frequency , genotype , genotyping , odds ratio , medicine , allele , oncology , tumor necrosis factor alpha , population , confidence interval , allele frequency , case control study , risk factor , immunology , cancer , gastroenterology , biology , gene , genetics , environmental health
Tumor necrosis factor a (TNFa) is an inflammatory cytokine that plays a crucial role in the immune response and the progression of cervical lesions. There is a growing body of data evaluating the value of a genetic variant in the TNFa gene with the risk of developing cervical cancer. The aim of this study was to explore the association of a variant, TNF‐308 G>A, residing in the TNFa gene with cervical cancer. A total of 91 women with cervical cancer and 161 women as the control group were recruited. DNA was extracted, and Taqman®‐probes‐based assay was used for genotyping. Our results showed that the minor allele frequency was 0.3 in total population, and the frequency of minor allele A was more in the case group compared with the control. The regression models in different genetic models also revealed that the allele A is a potential risk factor for the development of cervical cancer. In particular, in the dominant model, patients with AG and AA genotypes had a higher risk of developing cervical cancer with odds ratio (OR) of 2.75 (95% confidence interval [CI]: 1.57‐4.83, <0.001) and OR of 7.27 (95%CI: 2.5‐20.8, <0.001), compared with the GG genotype. Moreover, a similar outcome was obtained for smear test results. Our study demonstrated that TNF‐308 G>A located on TNF‐a was associated with the risk of cervical cancer, supporting further studies in a larger population and multicenter setting to show the value of emerging markers as risk stratification biomarkers in cervical cancer.