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Simulated microgravity reduces intracellular‐free calcium concentration by inhibiting calcium channels in primary mouse osteoblasts
Author(s) -
Sun Zhongyang,
Li Ying,
Zhou Hua,
Cai Min,
Liu Jing,
Gao Shanshan,
Yang Junsheng,
Tong Liangcheng,
Wang Jianling,
Zhou Sheng,
Hu Zebing,
Wang Yixuan,
Wang Ke,
Zhang Lijun,
Wang Han,
Zhang Lianchang,
Shi Fei,
Cao Xinsheng,
Zhang Shu,
Ji Yongzhang,
Zhao Jianning
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27685
Subject(s) - calcium , intracellular , calcium in biology , ryanodine receptor , extracellular , chemistry , microbiology and biotechnology , inositol trisphosphate , biophysics , voltage dependent calcium channel , osteoblast , calcium metabolism , t type calcium channel , calcium signaling , inositol , receptor , endocrinology , biochemistry , biology , in vitro , organic chemistry
Calcium homeostasis in osteoblasts plays fundamental roles in the physiology and pathology of bone tissue. Various types of mechanical stimuli promote osteogenesis and increase bone formation elicit increases in intracellular‐free calcium concentration in osteoblasts. However, whether microgravity, a condition of mechanical unloading, exerts an influence on intracellular‐free calcium concentration in osteoblasts or what mechanisms may underlie such an effect are unclear. Herein, we show that simulated microgravity reduces intracellular‐free calcium concentration in primary mouse osteoblasts. In addition, simulated microgravity substantially suppresses the activities of L‐type voltage‐sensitive calcium channels, which selectively allow calcium to cross the plasma membrane from the extracellular space. Moreover, the functional expression of ryanodine receptors and inositol 1,4,5‐trisphosphate receptors, which mediate the release of calcium from intracellular storage, decreased under simulated microgravity conditions. These results suggest that simulated microgravity substantially reduces intracellular‐free calcium concentration through inhibition of calcium channels in primary mouse osteoblasts. Our study may provide a novel mechanism for microgravity‐induced detrimental effects in osteoblasts, offering a new avenue to further investigate bone loss induced by mechanical unloading.