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Baicalin attenuates myocardial ischemia‐reperfusion injury through Akt/NF‐κB pathway
Author(s) -
Luan Yun,
Sun Chao,
Wang Jue,
Jiang Wen,
Xin Qian,
Zhang Zhaohua,
Wang Yibiao
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27587
Subject(s) - baicalin , protein kinase b , pi3k/akt/mtor pathway , western blot , apoptosis , medicine , tumor necrosis factor alpha , downregulation and upregulation , reperfusion injury , ischemia , chemistry , endocrinology , pharmacology , biochemistry , gene , high performance liquid chromatography , chromatography
Background Baicalin can attenuate myocardial ischemia‐reperfusion (I/R) on damage. However, the mechanisms are still not fully understood. The study aimed to investigate the antiapoptosis and anti‐inflammatory effects of baicalin on myocardial I/R‐induced injury. Methods We established male rats I/R model, and baicalin was intragastric administration after ischemia onset. All experimental animals were randomly divided into five groups: group I, sham; group II, I/R; group III, 50 mg/kg; group IV, 100 mg/kg; and group V, 200 mg/kg baicalin. Postoperation, left ventricular (LV) function was recorded by transthoracic echocardiography. Myocardial infarct size, number of vessels and apoptosis were detected by histology and immunohistochemistry. Furthermore, the messenger RNA (mRNA) and protein levels of tumor necrosis factor α (TNF‐α), interleukin 1β (IL‐1β), IL‐6, IL‐8, IL‐10, Bcl2, Bax, caspase‐3, phosphatidylinositol 3‐kinase (PI3K), Akt, p‐Akt, and nuclear factor‐κB (NF‐κB) p65 in myocardial tissues were measured by quantitative real‐time polymerase chain reaction and Western blot analysis assays. Result When compared with I/R groups, baicalin could significantly improve LV hemodynamic parameters. Myocardial infarct size and apoptosis were significantly decreased, but the vessel density was increased. The mRNA levels of TNF‐α, IL‐1β, IL‐6, and IL‐8 were downregulated, but the levels of IL‐10, proapoptotic genes caspase‐3, and the ratio of Bax/Bcl2 were upregulated. Moreover, the protein expression of PI3K, p‐Akt, and Akt were upregulated but NF‐κB p65 was downregulated in the groups III, IV, and V than in group II. Conclusion Our current study suggested that baicalin attenuated myocardial I/R‐induced damage, inhibited myocardial apoptosis, and inflammation by activating PI3K/Akt but suppressing NF‐κB signaling.