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Effects of calpain inhibitor on the apoptosis of hepatic stellate cells induced by calcium ionophore A23187
Author(s) -
Li Yanan,
Yan Yu,
Liu Fang,
Wang Luguang,
Feng Fumin,
Xiao Yonghong
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27478
Subject(s) - calpain , endoplasmic reticulum , apoptosis , microbiology and biotechnology , calcium , calcium in biology , hepatic stellate cell , cytoplasm , ionophore , intracellular , chemistry , calcium signaling , unfolded protein response , signal transduction , programmed cell death , endocrinology , biology , biochemistry , enzyme , organic chemistry
We previously showed that changes in calcium concentrations were related to cell apoptosis in vitro. The endoplasmic reticulum (ER) is the main component of calcium storage and signal transduction, and disrupting the balance of intracellular Ca 2+ can cause endoplasmic reticulum stress (ERS). In this process, the ER releases stored Ca 2+ into the cytoplasm and activates calpain‐2. To further investigate the effect of calpain in hepatic stellate cells (HSCs), in the current study, we examine the effect of N ‐acetyl‐leu‐leu‐norleucinal (ALLN) on apoptosis resulting from calcium ionophore A23187–induced ERS. Our findings indicate that calpain inhibition reduces calcium ionophore A23187–induced apoptosis of HSCs and decreases the expression of ER stress proteins that may be related to the calpain/caspase signaling pathway.