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A simple and highly efficient method for transduction of human adipose‐derived mesenchymal stem cells
Author(s) -
Bolandi Zohreh,
Hosseini Rad Seyed Mohammad Ali,
Soudi Sara,
Hashemi Seyed Mahmoud,
Ghanbarian Hossein
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27453
Subject(s) - mesenchymal stem cell , adipose tissue , microbiology and biotechnology , simple (philosophy) , transduction (biophysics) , signal transduction , chemistry , biology , biochemistry , philosophy , epistemology
Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into a wide range of cell types and provide a potential to transfer therapeutic protein in vivo, making them valuable candidates for gene therapy and cell therapy. However, using MSCs in in vivo is limited due to the low rate of transfection and transduction efficacy. Therefore, developing methods to efficiently transfer genes into MSCs would provide a number of opportunities for using them in the clinic. Here, we introduce a simple and robust method for efficient transduction of human adipose‐derived MSCs by modification under the culture condition of human embryonic kidney cells 293 (HEK293T) and MSCs. Moreover, as a transduction enhancer, polybrene was replaced with Lipofectamine, a cationic lipid. Therefore, we showed that transduction of primary cells can be increased efficiently by modifying the culture condition.