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The effect of ICRT‐3 on Wnt signaling pathway in head and neck cancer
Author(s) -
Sogutlu Fatma,
Kayabasi Cagla,
Ozmen Yelken Besra,
Asik Aycan,
Gasimli Roya,
Dogan Fatma,
Yilmaz Süslüer Sunde,
Biray Avcı Cigir,
Gunduz Cumhur
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27393
Subject(s) - wnt signaling pathway , biology , cell cycle , viability assay , apoptosis , cytotoxicity , signal transduction , cancer cell , cancer research , microbiology and biotechnology , cancer , biochemistry , genetics , in vitro
The effect of Wnt pathway in head and neck cancer could not be elucidated, even though the aberrant Wnt signaling plays a key role in the development of many types of cancer. The inhibitor of β‐catenin responsive transcription (ICRT‐3) blocks the Wnt signaling pathway by binding to β‐catenin, which is a coactivator of the Wnt signaling pathway and a promising agent for inhibiting aberrant signaling. In our study, we aimed to evaluate the effect of ICRT‐3 on the cytotoxicity, apoptosis, cell cycle progression, migration, and gene expressions in head and neck cancer stem cell (HNCSC) and hypopharynx cancer. The effect of this compound on cytotoxicity and cell viability in FaDu and HNCSC line was assessed by using the water‐soluble tetrazolium salt‐1 method. The effect of ICRT‐3 on apoptosis was detected by using Annexin V and caspase‐3, caspase‐9 kit, on cell cycle progression by cycle test plus DNA reagent kit, on gene expression by dual luciferase reporter assay, and on migration activity by wound healing assay in both cell lines. ICRT‐3 was determined to have cytotoxic and apoptotic effect in both cell lines. In addition, it was also found that the administration of ICRT‐3 caused cell cycle arrest and significant decrease in gene expression level and migration ability of the cells.