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The expression levels of long noncoding RNAs lnc0640 and lnc5150 and its gene single‐nucleotide polymorphisms in rheumatoid arthritis patients
Author(s) -
Zhang TianPing,
Zhang Qin,
Wu Jun,
Zhao YuLan,
Wang JieBing,
Leng RuiXue,
Fan YinGuang,
Li XiaoMei,
Pan HaiFeng,
Ye DongQing
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27346
Subject(s) - single nucleotide polymorphism , rheumatoid arthritis , genotype , genotyping , snp , peripheral blood mononuclear cell , taqman , medicine , erythrocyte sedimentation rate , immunology , allele , gene , rheumatoid factor , biology , microbiology and biotechnology , real time polymerase chain reaction , genetics , in vitro
Objectives The aim of our study was to evaluate two lncRNAs (lnc0640 and lnc5150) expressions and gene single‐nucleotide polymorphisms (SNPs) in rheumatoid arthritis (RA) patients. Methods The expressions of lncRNAs in peripheral blood mononuclear cells (PBMCs) were examined by quantitative real‐time reverse transcription polymerase chain reaction from 65 RA patients and 54 controls. Simultaneously, three SNPs (rs13039216, rs6085189, and rs6085190) of lnc0640, three SNPs (rs1590666, rs141561256, and rs144047453) of lnc5150 were genotyped using TaqMan SNP‐genotyping assays in 627 RA patients and 590 controls. Results The lnc0640 level in PBMCs from RA patients was significantly increased ( P = 0.001), whereas the lnc5150 level was significantly reduced ( P < 0.001) compared to controls. There were significant associations of lnc0640 and lnc5150 levels with C‐reactive protein in RA patients ( P = 0.011 and P = 0.014, respectively), while lnc5150 level was associated with erythrocyte sedimentation rate ( P = 0.022). TT genotype of rs13039216 in lnc0640 gene was statistically associated with a reduced risk of RA (TT vs CC; P = 0.046), and a decreased risk of rs13039216 variant was observed under the recessive model ( P = 0.038). In addition, the G allele of rs141561256 polymorphism in lnc5150 gene was significantly associated with rheumatoid factor in RA patients ( P = 0.034). There were no associations between lnc0640 and lnc5150 levels and their respective genotype in RA patients. Conclusions The expressions of lnc0640 and lnc5150 were alternated in the RA patients, suggesting that these lncRNAs may involve in the development of RA.