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Apoptotic effect of resveratrol on human T‐ALL cell line CCRF‐CEM is unlikely exerted through alteration of BAX and BCL2 promoter methylation
Author(s) -
Zadi Heydarabad Milad,
Vatanmakanian Mousa,
Abdolalizadeh Jalal,
Mohammadi Hamed,
Azimi Ako,
Mousavi Ardehaie Reza,
Movasaghpour Aliakbar,
Farshdousti Hagh Majid
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27333
Subject(s) - resveratrol , dna methylation , methylation , apoptosis , cancer research , downregulation and upregulation , biology , promoter , epigenetics , gene , microbiology and biotechnology , leukemia , cell culture , programmed cell death , gene expression , chemistry , pharmacology , immunology , genetics
One of the fundamental barriers leading to failure of leukemia therapy is the resistance against conventional chemotherapies, common modality used to cure leukemia. Having the potential to trigger apoptosis in various human leukemia cell lines, resveratrol is regarded as a robust agent in chemotherapy regimens. The current study was aimed to assess whether the apoptotic effect of resveratrol on T‐cell acute lymphoblastic leukemia cell line, CCRF‐CEM, is exerted through DNA methylation of BAX and BCL2 gene promoters. For this purpose, the CCRF‐CEM cells were treated by resveratrol under standard cell culture. To analyze the promoter DNA methylation changes, we used methylation‐specific polymerase chain reaction technique following the resveratrol treatment at different dosages and time intervals. Based on our previous study, the resveratrol treatment can trigger apoptosis in CCRF‐CEM cell line via upregulation of apoptotic BAX gene and downregulation of antiapoptotic BCL2 gene. Despite these alterations in gene expression, the current study reveals no changes in DNA methylation patterns of subjected genes following the resveratrol treatment. Unchanged status of DNA methylation of BAX and BCL2 genes may suggest that resveratrol causes the gene expression changes through a distinct mechanism which requires further studies to be understood.