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MiR‐99a‐5p inhibits bladder cancer cell proliferation by directly targeting mammalian target of rapamycin and predicts patient survival
Author(s) -
Liu Yan,
Li Bingxun,
Yang Xianxu,
Zhang Chenglong
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27318
Subject(s) - bladder cancer , pi3k/akt/mtor pathway , p70 s6 kinase 1 , cancer , cancer research , cancer cell , malignancy , cell growth , medicine , biology , signal transduction , microbiology and biotechnology , genetics
Bladder cancer is a common malignancy and miR‐99a‐5p has been reported to be downregulated in bladder cancer, but its function and the underlying mechanism in bladder cancer development remains largely unclear. Here, we report that miR‐99a‐5p expression was decreased in bladder cancer compared with the adjacent normal tissues. Receiver operating characteristic curve revealed that miR‐99a‐5p expression signature had area under curve value of 0.7989 in differing bladder cancer from the adjacent normal tissues. Bladder cancer patients with low expression of miR‐99a‐5p had a poor survival rate. Gain‐of‐function and loss‐of‐function approaches demonstrated that miR‐99a‐5p inhibited bladder cell proliferation and cell cycle. Furthermore, we identified that mammalian target of rapamycin (mTOR) was a direct target of miR‐99a‐5p and mTOR restore could rescue the proliferative ability of bladder cancer cells. Moreover, miR‐99a‐5p/mTOR axis regulated S6K1 phosphorylation. These suggested that miR‐99a‐5p/mTOR axis might be a therapeutic target for bladder cancer.