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Effects of telomerase inhibitor on epigenetic chromatin modification enzymes in malignancies
Author(s) -
Biray Avci Cigir,
Dogan Fatma,
Ozates Ay Neslihan Pinar,
Goker Bagca Bakiye,
Abbaszadeh Zeka,
Gunduz Cumhur
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27301
Subject(s) - telomerase , epigenetics , cancer research , biology , chromatin , apoptosis , cell culture , myeloid leukemia , telomerase reverse transcriptase , microbiology and biotechnology , gene , biochemistry , genetics
Telomerase has a critical role in cell proliferation, tumor maintaining, and therapy resistance, which act by modifying many signaling pathways. 2‐[(E)‐3‐Naphtalen‐2‐yl‐but‐2‐enoylamino]‐benzoic acid (BIBR1532) is one of the most studied telomerase inhibitors, and it targets telomerase components TERC and TERT. In this novel study, we aimed to investigate the epigenetic effects of BIBR1532 on both hematologic malignancies and solid tumors. K‐562 human chronic myeloid leukemia cell line and U87MG glioblastoma cell line were compared with control groups without BIBR1532 treatment. Cytotoxic effects of BIBR1532 were determined by using WST‐1 assay. Apoptotic effects of BIBR1532 were detected by using annexin V method. To assess expression changes in the human epigenetic chromatin modification enzyme genes, total RNA was isolated from K‐562 and U87MG cells treated with BIBR1532 and untreated control cells. BIBR1532 induced 2.41‐fold apoptotic cell death in U87MG cell lines compared with control groups. Apoptosis was slightly induced in K‐562 cells with BIBR1532 treatment compared with control cells. We observed that BIBR1532 also regulates similar genes in both cell lines, and it is useful on epigenetic mechanisms. As a result, telomerase inhibitor BIBR1532 has a significant effect on both hematological malignancies and solid tumors.