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Retracted : Downregulation of long noncoding RNA Sox2ot protects PC‐12 cells from hydrogen peroxide–induced injury in spinal cord injury via regulating the miR‐211‐myeloid cell leukemia‐1 isoform2 axis
Author(s) -
Yin Dong,
Zheng Xiaoqing,
Zhuang Jianxiong,
Wang Liangze,
Liu Bin,
Chang Yunbing
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27280
Subject(s) - pi3k/akt/mtor pathway , downregulation and upregulation , p70 s6 kinase 1 , viability assay , microbiology and biotechnology , protein kinase b , chemistry , transfection , apoptosis , biology , cancer research , signal transduction , biochemistry , gene
This study aimed to investigate the effects and possible mechanisms of long noncoding RNA (lncRNA) Sox2 overlapping transcript (Sox2ot) on hydrogen peroxide (H 2 O 2 )‐induced injury in pheochromocytoma (PC‐12) cells. PC‐12 cells were treated with H 2 O 2 to cell injury. The cells were transfected with short‐hairpin RNA directed against Sox2ot (sh‐Sox2ot), small interfering RNA directed against myeloid cell leukemia‐1 (MCL‐1) isoform2 (si‐MCL‐1), a miR‐211 mimic, a miR‐211 inhibitor, and their negative controls. Under different transfected treatments, cell viability, migration, invasion, and apoptosis as well as the expressions of apoptosis‐ and autophagy‐related proteins were investigated. Besides, the regulatory relationships between Sox2ot and miR‐211, miR‐211 and MCL‐1, as well as between MCL‐1 and the protein kinase B (Akt)/mammalian target of the rapamycin (mTOR)/p70 ribosomal S6 protein kinase (p70S6K) signaling pathway were explored. Suppression of Sox2ot inhibited H 2 O 2 ‐induced PC‐12 cell injury by increasing cell viability, migration, invasion, and decreasing apoptosis and autophagy. Moreover, suppression of Sox2ot increased miR‐211 expression and alleviated H 2 O 2 ‐induced injury in PC‐12 cells possibly via upregulation of miR‐211. Furthermore, MCL‐1 isoform2 was identified as a direct target of miR‐211 and could be negatively regulated by miR‐211. Suppression of miR‐211 aggravated H 2 O 2 ‐induced cell injury by regulation of MCL‐1 isoform2. Besides, inhibition of miR‐211 suppressed the activation of the Akt/mTOR/p70S6K signaling pathway in H 2 O 2 ‐treated PC‐12 cells, which was reversed after knockdown of MCL‐1 isoform2 at the same time. Our findings indicate that downregulation of Sox2ot may protect PC‐12 cells from H 2 O 2 ‐induced injury in SCI via targeting the miR‐211/MCL‐1 isoform2 axis. MCL‐1 isoform2 may further regulate the activation of the Akt/mTOR/p70S6K pathway to mediate H 2 O 2 ‐induced injury. The Sox2ot‐miR‐211‐MCL‐1 isoform2 axis may be a promising therapeutic strategy for SCI.