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Retracted : Platelet‐derived growth factor subunit B is required for tendon‐bone healing using bone marrow–derived mesenchymal stem cells after rotator cuff repair in rats
Author(s) -
Wang LinLiang,
Yin XueFeng,
Chu XiuCheng,
Zhang YongBing,
Gong XiaoNan
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27143
Subject(s) - mesenchymal stem cell , platelet derived growth factor receptor , medicine , bone marrow , stem cell , growth factor , pathology , cancer research , microbiology and biotechnology , biology , receptor
As a common cause of shoulder pain and disability, rotator cuff injury (RCI) represents a debilitating condition affecting an individual’s quality of life. Although surgical repair has been shown to be somewhat effective, many patients may still suffer from reduced shoulder function. The aim of the current study was to identify a more effective mode of RCI treatment by investigating the effect of platelet‐derived growth factor subunit B (PDGF‐B) on tendon‐bone healing after RCI repair by modifying bone marrow–derived mesenchymal stem cells (BMSCs). Surface markers of BMSCs were initially detected by means of flow cytometry, followed by establishment of the rat models and construction of the lentiviral vector. 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2‐H‐tetrazolium bromide, Thiazolyl Blue Tetrazolium Bromide (MTT) assay, alizarin red staining, and oil red O staining were used to provide verification that PDGF‐B was indeed capable of promoting BMSC viability, osteogenic and adipogenic differentiation capability. Furthermore, biomechanical assessment results indicated that PDGF‐B could increase the ultimate load and stiffness of the tendon tissue. Real‐time reverse‐transcription quantitative polymerase chain reaction and Western blot analysis methods provided evidence suggesting that PDGF‐B facilitated the expression of tendon‐bone healing–related genes and proteins, while contrasting results were obtained after PDGF‐B silencing. Taken together, the key findings of the current study provided evidence suggesting that overexpressed PDGF‐B could act to enhance tendon‐bone healing after RCI repair, thus highlighting the potential of the functional promotion of PDGF‐B as a future RCI therapeutic approach.