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The establishment of clonally derived chicken embryonic fibroblast cell line (CSC) with high transfection efficiency and ability as a feeder cell
Author(s) -
Zhao Ruifeng,
Jin Jing,
Sun Xinyu,
Jin Kai,
Wang Man,
Ahmed Mahmoud F.,
Zuo Qisheng,
Zhang Yani,
Zhao Zhenhua,
Chen Guohong,
Li Bichun
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27137
Subject(s) - transfection , embryonic stem cell , cell culture , fibroblast , subculture (biology) , cell , microbiology and biotechnology , biology , embryo , genetics , gene
Abstract This study established a single cloned chicken embryonic fibroblast (CEF) cell line. It solves the main problem of the instability of a cultured primary cell and its impact on the experiment. In this study, CEF pass through this crisis and formed a continuous cell line after subculture. We isolated single postcrisis CEF by a mouth pipette under a convert microscope then established a single cloned cell line named CSC‐1‐5 which passaged continuously from 96‐well plates to 60 mm culture plates. CSC has a normal chicken diploid karyotype, no tumorigenicity, and a high G2/M phase cell ratio. We found that Fugene could mediate the transfection of CSCs efficiently; it was significantly improved compared with the primary cells. It could also promote the proliferation of chicken embryonic stem cell as a feeder layer.