Premium
Expression of microRNAs and IRAK1 pathway genes are altered in gastric cancer patients with Helicobacter pylori infection
Author(s) -
Ranjbar Reza,
Hesari AmirReza,
Ghasemi Faezeh,
Sahebkar Amirhossein
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27067
Subject(s) - microrna , gene , cancer , helicobacter pylori , pten , biology , cancer research , helicobacter pylori infection , signal transduction , genetics , pi3k/akt/mtor pathway
Gastric cancer (GC) is among the most common cancer types in the world and one of the most lethal gastrointestinal cancers. MicroRNAs (miRNAs) can be of great importance in the early detection of GC. This study aimed to investigate some miRNAs and the genes involved in IRAK1 pathways in the serum of GC patients with Helicobacter pylori (H. pylori) infections compared to the control group. Total RNA was extracted from the serum of GC patients with H. pylori infection and healthy volunteers. The expression levels of miRNAs and the genes were assessed using Real time RT‐PCR with specific primers. Our data showed that miR‐146, miR‐375, and Let‐7 were down‐regulated and miR‐19 and miR‐21 were up‐regulated in GC patients with H. pylori infection. Other genes involved in the pathways such as RAS , MYC , NFKB , JUN , TRAF6 , and IRAK4 were overexpressed; while the expression of PTEN gene was decreased compared to the control group. Expression of miRNAs and IRAK1 pathway genes are altered in patients with GC and H. pylori infection. This suggests a potential role for the above‐mentioned miRNAs and genes in the diagnosis of GC.