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HSP‐enriched properties of extracellular vesicles involve survival of metastatic oral cancer cells
Author(s) -
Ono Kisho,
Eguchi Takanori,
Sogawa Chiharu,
Calderwood Stuart K.,
Futagawa Junya,
Kasai Tomonari,
Seno Masaharu,
Okamoto Kuniaki,
Sasaki Akira,
Kozaki Kenichi
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.27039
Subject(s) - gene knockdown , heat shock protein , secretion , cancer research , lymph node , cancer , biology , lymph , cancer cell , hsp90 , heat shock , microvesicles , medicine , apoptosis , pathology , immunology , microrna , gene , endocrinology , biochemistry
Cancer cells often secrete extracellular vesicles (EVs) that carry heat shock proteins (HSPs) with roles in tumor progression. Oral squamous cell carcinoma (OSCC) belongs to head and neck cancers (HNC) whose lymph‐node‐metastases often lead to poor prognosis. We have examined the EV proteome of OSCC cells and found abundant secretion of HSP90‐enriched EVs in lymph‐node‐metastatic OSCC cells. Double knockdown of HSP90α and HSP90β, using small interfering RNA significantly reduced the survival of the metastatic OSCC cells, although single knockdown of each HSP90 was ineffective. Elevated expression of these HSP90 family members was found to correlate with poor prognosis of HNC cases. Thus, elevated HSP90 levels in secreted vesicles are potential prognostic biomarkers and therapeutic targets in metastatic OSCC.