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HOTAIR enhanced paclitaxel and doxorubicin resistance in gastric cancer cells partly through inhibiting miR‐217 expression
Author(s) -
Wang Hui,
Qin Rong,
Guan Aoran,
Yao Ying,
Huang Yun,
Jia Hongping,
Huang Weikang,
Gao Jianpeng
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26901
Subject(s) - hotair , paclitaxel , ectopic expression , cancer research , cell cycle , downregulation and upregulation , biology , cancer cell , cancer , cell growth , cell , chemistry , cell culture , long non coding rna , gene , biochemistry , genetics
Drug resistance is a big obstacle for clinical anti‐tumor treatment outcome. However, the role of HOTAIR in drug resistance in gastric cancer (GC) remains unknown. In this study, we showed that overexpression of HOTAIR enhanced paclitaxel and doxorubicin resistance in GC cells. Furthermore, the expression of HOTAIR was upregulated in GC tissues and higher expression of HOTAIR was associated with late stage. In addition, we showed that miR‐217 expression was lower in GC tissues compared with the paired non‐tumour tissues and downregulated expression of miR‐217 was correlated with late stage. Interestingly, the expression of miR‐217 was negatively correlated with HOTAIR expression in GC tissues. Ectopic expression of HOTAIR increased GC cell proliferation, cell cycle, and migration. Elevated expression of HOTAIR suppressed miR‐217 expression and enhanced GPC5 and PTPN14 expression. Furthermore, we demonstrated that overexpression of miR‐217 suppressed paclitaxel and doxorubicin resistance in GC cells. Ectopic expression of HOTAIR promoted drug resistance and increased GC cell proliferation, cell cycle, and migration by targeting miR‐217. These data suggested that overexpression of HOTAIR enhanced paclitaxel and doxorubicin resistance in GC cells through inhibiting miR‐217 expression.