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Role of miR‐486‐5p in regulating renal cell carcinoma cell proliferation and apoptosis via TGF‐β–activated kinase 1
Author(s) -
He Yanfa,
Liu Jianzhen,
Wang Yongjun,
Zhu Xiaoli,
Fan Zhengchao,
Li Chongbin,
Yin Hang,
Liu Ying
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26900
Subject(s) - apoptosis , cancer research , transforming growth factor , renal cell carcinoma , cell growth , kinase , microbiology and biotechnology , chemistry , medicine , biology , biochemistry
Renal cell carcinoma (RCC) is a common kidney tumor in adults. The role of miR‐486‐5p in RCC is unknown. The aim of our study was to identify new targets regulated by miR‐486‐5p in RCC, to obtain a deeper insight into the network and to better understand the role of these microRNAs and their targets in carcinogenesis of RCC. We performed a series of tests and found consistently lower expression levels of miR‐486‐5p in kidney cancer cells. Restoration of miR‐486‐5p expression in RCC cells could lead to the suppression of cell proliferation and the increase of cell apoptosis. Further studies demonstrated that TGF‐β–activated kinase 1 was a target gene of miR‐486‐5p in kidney cancer cells. It was also shown that C‐C motif chemokine ligand 2 (CCL2) from tumor‐associated macrophages downregulated miR‐486‐5p expression, and miR‐486‐5p inhibited RCC cell proliferation and apoptosis resistance induced by CCL2. The study demonstrates that there are potential diagnosis and therapy values of miR‐486‐5p in RCC.