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Knockdown of Uba2 inhibits colorectal cancer cell invasion and migration through downregulation of the Wnt/β‐catenin signaling pathway
Author(s) -
Cheng Hongjing,
Sun Xun,
Li Ji,
He Ping,
Liu Wanqi,
Meng Xiangwei
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26890
Subject(s) - gene knockdown , wnt signaling pathway , colorectal cancer , cancer research , epithelial–mesenchymal transition , metastasis , cancer , signal transduction , mouse model of colorectal and intestinal cancer , biology , cell growth , cancer cell , medicine , oncology , cell culture , microbiology and biotechnology , genetics
Colorectal cancer is a serious threat to human health, and has a high mortality rate. There is currently no effective therapy for end‐stage colorectal cancer. In recent years, molecular targeted therapy has received increasing attention for cancer treatment. In particular, the role of Uba2, a vital component of SUMO‐activating enzyme, has been highlighted, which plays important roles in the progression of certain cancers; however, its role in colorectal cancer remains unclear. Accordingly, the aim of this study was to evaluate the relationship between Uba2 and colorectal cancer. Uba2 expression was knocked down in two colorectal cancer cell lines, and gene microarray analysis was conducted, followed by proliferation, migration, and invasion assays. Uba2 knockdown influenced the expression of several genes, and significantly inhibited the proliferation, migration, and invasion of cancer cells. To determine the underlying mechanism, the expression of related signaling pathways and molecules was evaluated in the knockdown cell lines. Overall, the results suggest that Uba2 participates in the progression, invasion, and metastasis of colorectal cancer, and the possible mechanism is via regulating the Wnt signaling pathway and enhancing epithelial‐mesenchymal transition behaviors of colorectal cancer cells. Therefore, Uba2 is expected to be an important oncoprotein and potential therapeutic target in colorectal cancer.