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microRNA‐665 promotes the proliferation and matrix degradation of nucleus pulposus through targeting GDF5 in intervertebral disc degeneration
Author(s) -
Tan Hongyu,
Zhao Liang,
Song Ruipeng,
Liu Yilin,
Wang Limin
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26888
Subject(s) - degeneration (medical) , intervertebral disc , nucleus , matrix (chemical analysis) , degenerative disc disease , microbiology and biotechnology , extracellular matrix , chemistry , anatomy , medicine , pathology , biology , lumbar , chromatography
Growing evidences suggested that microRNAs (miRNAs) played important roles in the development of intervertebral disc degeneration (IDD). However, the expression level and function of miR‐665 in IDD remain unknown. In this study, we showed that the expression level of miR‐665 was upregulated in degenerative human NP samples. In addition, miR‐665 expression level gradually increased with the exacerbation of disc degeneration grade. Moreover, miR‐665 expression level was positively associated with the Pfirrmann grade. Ectopic expression of miR‐665 promoted NP cell growth. Furthermore, miR‐665 overexpression decreased aggrecan and Col II expression and ectopic expression of miR‐665 increased MMP‐3 and MMP‐13 expression in NP cell. We identified growth differentiation factor 5 (GDF5) was a direct target gene of miR‐665 in NP cell and enforced expression of miR‐665 decreased GDF5 expression. Elevated expression of miR‐665 enhanced NP cell proliferation and decreased aggrecan and Col II expression. In addition, ectopic expression of miR‐665 increased MMP‐3 and MMP‐13 expression through inhibiting GDF5 expression in NP cells. These results suggested that dysregulated miR‐665 expression might act an important role in the development of IDD.