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Competing endogenous RNA network crosstalk reveals novel molecular markers in colorectal cancer
Author(s) -
Samir Nehal,
Matboli Marwa,
ElTayeb Hanaa,
ElTawdi Ahmed,
Hassan Mohmed K.,
Waly Amr,
ELAkkad Hesham A. E.,
Ramadan Mohamed G.,
AlBelkini Tarek N.,
ElKhamisy Sherif,
ElAsmar Farid
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26884
Subject(s) - competing endogenous rna , microrna , biomarker , colorectal cancer , rna , biology , long non coding rna , gene , cancer research , in silico , cancer , computational biology , genetics
Abstract The competing endogenous RNA networks play a pivotal role in cancer diagnosis and progression. Novel properstrategies for early detection of colorectal cancer (CRC) are strongly needed. We investigated a novel CRC‐specific RNA‐based integrated competing endogenous network composed of lethal3 malignant brain tumor like1 (L3MBTL1) gene, long non‐coding intergenic RNA‐ ( lncRNA RP11‐909B2.1 ) and homo sapiens microRNA‐595 (hsa‐ miRNA‐595 ) using in silico data analysis. RT‐qPCR‐based validation of the network was achieved in serum of 70 patients with CRC, 40 patients with benign colorectal neoplasm, and 20 healthy controls. Moreover, in cancer tissues of 20 of the 70 CRC cases were involved in the study. The expression of RNA‐based biomarker network in both CRC and adjacent non‐tumor tissues and their correlation with the serum levels of this network members was investigated. Lastly, the expression levels of the chosen ceRNA was verified in CRC cell line. Our results revealed that the three RNAs‐based biomarker network (long non‐coding intergenic RNA‐[ lncRNA RP11‐909B2.1 ], Homo sapiens microRNA‐595 [hsa‐ miRNA‐595 ], and L3MBTL1 mRNA ), had high sensitivity and specificity for discriminating CRC from healthy controls and also from benign colorectal neoplasm. The data suggest that among these three RNAs, serum lncRNA RP11‐909B2.1 could be a promising independent prognostic factors in CRC. The circulatory RNA based biomarker panel can act as potential biomarker for CRC diagnosis and prognosis.