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Genetic polymorphisms in the FVII gene is associated with lower extremity deep venous thrombosis: A case‐control study
Author(s) -
Liu JianWei,
Chen DongQiong
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26860
Subject(s) - genotype , fibrinogen , medicine , venous thrombosis , body mass index , case control study , factor vii , allele , restriction fragment length polymorphism , gastroenterology , endocrinology , thrombosis , immunology , gene , biology , genetics , coagulation
This study aims to explore the associations between FVII gene polymorphisms (R353Q, 5′F7, and −402G/A) and lower extremity deep venous thrombosis (LEDVT) in a Chinese Han population. LEDVT patients (153) and healthy people (174) were, respectively, as case and control groups and evaluated related biochemical indicators. Gene polymorphisms of R353Q, 5′F7, and −402G/A of FVII, serum FVII level, antithrombin activity, plasma fibrinogen content, and plasma D‐dimer (D‐D) level were determined by polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP), ELISA, chromogenic substrate assay, coagulating assay, and Immunoturbidimetry assay, respectively. Compared with the control group, the case group had a higher level of body mass index (BMI), glucose, and fibrinogen, and lower level of total cholesterol (TC). Notable differences were found in GG genotype, G and A alleles, as well as distribution of recessive model of −402G/A. The serum FVII level of GG genotype was higher than that of GA and AA genotypes. FIB and D‐D had a higher level had a lower level in GG genotype when compared with GA and AA genotypes. Smoking, drinking, serum FVII level, and −402G/A‐GG were the independent risk factors for LEDVT. This study demonstrates that −402G/A of FVII may be a risk factor for LEDVT patients in a Chinese Han population.