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A siRNA‐based method for efficient silencing of PYROXD1 gene expression in the colon cancer cell line HCT116
Author(s) -
Shabani Samira,
Mahjoubi Frouzandeh,
Moosavi Mohammad A.
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26858
Subject(s) - gene silencing , apoptosis , gene knockdown , cell cycle , transfection , small interfering rna , rna interference , flow cytometry , microbiology and biotechnology , cancer research , colorectal cancer , cell culture , biology , cell growth , chemistry , cancer , gene , rna , biochemistry , genetics
Purpose The aim of this study was to determine the biological function of pyridine nucleotide‐disulfide oxidoreductase domain 1 ( PYROXD1 ), a recently discovered protein, in colon cancer cell line HCT116. Methods The small interfering RNA (siRNA) was designed rationally on the basis of the target sequence against PYROXD1. Relative PYROXD1 mRNA levels were measured by a quantitative real‐time polymerase chain reaction. Flow cytometry was performed to monitor tumor cells proliferation and apoptosis after siRNA transfection. Results Knockdown of PYROXD1 arrested the cell cycle, and induced late apoptosis in colon cancer cell line HCT116 Discussion Taken together, these results revealed the critical roles of PYROXD1 in regulating cell cycle and apoptosis and possibly will signify its therapeutic potential for targeting colorectal cancer models.