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Human APOBEC3B interacts with the heterogenous nuclear ribonucleoprotein A3 in cancer cells
Author(s) -
Mishra Nawneet,
Reddy K Sony,
Timilsina Uddhav,
Gaur Deepak,
Gaur Ritu
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26855
Subject(s) - ribonucleoprotein , biology , immunoprecipitation , cytidine deaminase , downregulation and upregulation , stable isotope labeling by amino acids in cell culture , rna , cancer research , apobec , microbiology and biotechnology , somatic hypermutation , cancer , proteomics , genome , genetics , cell culture , gene , antibody , b cell
Human APOBEC3B (A3B), like other APOBEC3 members, is a cytosine deaminase which causes hypermutation of single stranded genome. Recent studies have shown that A3B is predominantly elevated in multiple cancer tissues and cell lines such as the bladder, cervix, lung, head and neck, and breast. Upregulation and activation of A3B in developing tumors can cause an unexpected cluster of mutations which promote cancer development and progression. The cellular proteins which facilitate A3B function through direct or indirect interactions remain largely unknown. In this study, we performed LC‐MS‐based proteomics to identify cellular proteins which coimmunoprecipitated with A3B. Our results indicated a specific interaction of A3B with hnRNP A3 (heterogeneous nuclear ribonucleoprotein). This interaction was verified by co‐immunoprecipitation and was found to be RNA‐dependent. Furthermore, A3B and hnRNP A3 colocalized as evident from immunofluorescence analysis.