Premium
Structure, molecular dynamics simulation, and docking studies of Dictyostelium discoideum and human STRAPs
Author(s) -
Kumar Rakesh,
Saran Shweta
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26840
Subject(s) - dictyostelium discoideum , docking (animal) , microbiology and biotechnology , dictyostelium , molecular dynamics , chemistry , biology , computational biology , biochemistry , medicine , computational chemistry , nursing , gene
The Serine Threonine kinase Receptor Associated Protein (STRAP) is a WD40 containing protein that provides a platform for protein interactions during cell proliferation and development. Overexpression and misregulation of STRAP contributes to various carcinomas that are now recognized as therapeutic targets especially for colorectal and lung cancers. The present study was undertaken to find an effective drug against this molecule using a simple system like Dictyostelium discoideum ; which shares close homology to humans. Using techniques like structural modeling, molecular dynamics (MD) simulation and molecular docking, we found similar structure and dynamic behaviors in both, except for the presence of dissimilar numbers of β‐sheets and loop segments. We identified a novel and potential drug targeted to STRAP. The results obtained allow us to use Dictyostelium as a model system for further in vivo studies. Finally, the results of protein‐protein interactions using molecular docking and essential dynamics studies show STRAP to participate in TGF‐β signaling in humans. Further, we show some structural units that govern the interaction of TGFβ‐RI with STRAP and Smad7 proteins in TGF‐β signaling pathway. In conclusion, we propose that D. discoideum can be used for enhancing our knowledge about STRAP protein.