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Cardiomyogenic differentiation of human adipose‐derived mesenchymal stem cells transduced with Tbx20 ‐encoding lentiviral vectors
Author(s) -
Neshati Vajiheh,
Mollazadeh Samaneh,
Fazly Bazzaz Bibi Sedigheh,
de Vries Antoine AF,
Mojarrad Majid,
NaderiMeshkin Hojjat,
Neshati Zeinab,
Kerachian Mohammad Amin
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26818
Subject(s) - mesenchymal stem cell , microbiology and biotechnology , stem cell , viral vector , regenerative medicine , cellular differentiation , biology , genetics , gene , recombinant dna
Ischemic heart disease often results in myocardial infarction and is the leading cause of mortality and morbidity worldwide. Improvement in the function of infarcted myocardium is a main purpose of cardiac regenerative medicine. One possible way to reach this goal is via stem cell therapy. Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of cell types but display limited cardiomyogenic differentiation potential. Members of the T‐box family of transcription factors including Tbx20 play important roles in heart development and cardiomyocyte homeostasis. Therefore, in the current study, we investigated the potential of Tbx20 to enhance the cardiomyogenic differentiation of human adipose‐derived MSCs (ADMSCs). Human ADMSCs were transduced with a bicistronic lentiviral vector encoding Tbx20 (murine) and the enhanced green fluorescent protein ( eGFP ) and analyzed 7 and 14 days post transduction. Transduction of human ADMSCs with this lentiviral vector increased the expression of the cardiomyogenic differentiation markers ACTN1 , TNNI3 , ACTC1 , NKX2.5 , TBX20 (human), and GATA4 as revealed by RT‐qPCR. Consistently, immunocytological results showed elevated expression of α‐actinin and cardiac troponin I in these cells in comparison to the cells transduced with control lentiviral particles coding for eGFP alone. Accordingly, forced expression of Tbx20 exerts cardiomyogenic effects on human ADMSCs by increasing the expression of cardiomyogenic differentiation markers at the RNA and protein level.