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Exploring sand fly salivary proteins to design multiepitope subunit vaccine to fight against visceral leishmaniasis
Author(s) -
Pandey Rajan Kumar,
Prajapati Vijay Kumar
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26719
Subject(s) - antigenicity , virology , biology , visceral leishmaniasis , leishmania , immunogenicity , adjuvant , in silico , protein subunit , immunity , leishmaniasis , immunology , immune system , antigen , genetics , gene , computer science , parasite hosting , world wide web
Visceral leishmaniasis (VL) is caused by the parasites of Leishmania donovani complex, leads to the death of 20 000 to 40 000 people from 56 affected countries, worldwide. Till date, there is not a single available vaccine candidate to prevent the VL infection, and treatment only relies upon expensive and toxic chemotherapeutic options. Consequently, immunoinformatics approach was applied to design a multiepitope‐based subunit vaccine to enhance the humoral as well as cell‐mediated immunity. Constructed vaccine candidate was further subjected to evaluation on allergenicity and antigenicity and physiochemical parameters. Later on, disulfide engineering was performed to increase the stability of vaccine construct. Also, molecular docking and molecular dynamics simulation study were performed to check the binding affinity and stability of toll‐like receptor‐4 to vaccine construct complex. Finally, codon optimization and in silico cloning were performed to ensure the expression of proposed vaccine construct in a microbial expression system.