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MiR‐548a‐3p regulates inflammatory response via TLR4/NF‐κB signaling pathway in rheumatoid arthritis
Author(s) -
Wang Yingliang,
Zheng Feng,
Gao Guohong,
Yan Shushan,
Zhang Laixia,
Wang Li,
Cai Xiao,
Wang Xiaodong,
Xu Donghua,
Wang Jibo
Publication year - 2019
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26659
Subject(s) - microvesicles , microrna , pathogenesis , rheumatoid arthritis , tlr4 , signal transduction , medicine , peripheral blood mononuclear cell , immunology , microarray , inflammation , cancer research , nf κb , biology , gene , gene expression , microbiology and biotechnology , genetics , in vitro
Currently published studies have implicated that microRNAs (miRNAs) including exosomes‐encapsulated miRNAs play a critical role in rheumatoid arthritis (RA). Previously, we have found that exosomes‐encapsulated miR‐548a‐3p was significantly decreased in serum samples from RA patients by miRNAs microarray analysis. However, little is known of the role of miR‐548a‐3p in the development and progression of RA. In this study, we aim to investigate the underlying molecular mechanisms of miR‐548a‐3p in RA, which will provide new insight into understanding the pathogenesis of RA and identifying novel therapeutics targets for this disease. As validated by quantitative real‐time polymerase chain reaction (qRT‐PCR), the expression of miR‐548a‐3p in serum exosomes and peripheral blood mononuclear cells (PBMCs) of RA patients ( n = 76) was obviously down‐regulated compared with healthy controls ( n = 20). Serum exosomal miR‐548a‐3p was negatively associated with levels of CRP, RF, and ESR in serum of patients with RA. MiR‐548a‐3p could inhibit the proliferation and activation of pTHP‐1 cells by regulating the TLR4/NF‐κB signaling pathway. Accordingly, exosomes‐delivered miR‐548a‐3p may be a critical factor predicting the disease activity of RA. MiR‐548a‐3p/TLR4/NF‐κB axis can serve as promising targets for RA diagnosis and treatment.