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Decreased levels of serum exosomal miR‐638 predict poor prognosis in hepatocellular carcinoma
Author(s) -
Shi Min,
Jiang Ye,
Yang Lu,
Yan Shushan,
Wang YuGang,
Lu XiaoJie
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26650
Subject(s) - hepatocellular carcinoma , exosome , medicine , microrna , microvesicles , biomarker , proportional hazards model , downregulation and upregulation , cancer , stage (stratigraphy) , oncology , carcinoma , cancer research , biology , paleontology , biochemistry , gene
Currently available studies have implicated that exosome‐delivered microRNAs (miRNAs) play crucial roles in human cancer. However, the association of serum exosomal miR‐638 and hepatocellular carcinoma (HCC) remains largely unknown. We aim to investigate the expression of exosomal miR‐638 in serum of HCC patients and its prognostic role in this deadly disease. Kaplan‐Meier and Cox regression analyses were used to determine the survival of patients histologically diagnosed with HCC. Reduced levels of exosomal miR‐638 in serum samples from patients with HCC were identified by real‐time PCR. Negative association of serum exosomal miR‐638 with tumor size, vascular infiltration, and TNM stage was observed in HCC patients. Besides, the proliferation of Huh7 and SMCC7721 HCC cells were significantly inhibited when miR‐638 was over‐expressed in these cells. In addition, HCC patients with lower levels of serum exosomal miR‐638 had poor overall survival than those with higher levels of exosomal miR‐638 in serum. Our study strongly suggests that serum exosome‐delivered miR‐638 may serve as a novel circulating biomarker for HCC. Downregulation of miR‐638 predicts poor prognosis for patients with HCC.