Premium
Immortalization of canine adipose‐derived mesenchymal stem cells and their seminiferous tubule transplantation
Author(s) -
Fang Jia,
Wei Yudong,
Teng Xin,
Zhao Shanting,
Hua Jinlian
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26574
Subject(s) - mesenchymal stem cell , microbiology and biotechnology , immortalised cell line , adipose tissue , biology , seminiferous tubule , stem cell , transplantation , chondrogenesis , adipogenesis , immunology , cell , endocrinology , medicine , sertoli cell , spermatogenesis , genetics
Abstract Adipose‐derived mesenchymal stem cells (ADSCs) are proven to provide good effects in numerous tissue engineering application and other cell‐based therapies. However, the difficulty in the proliferation of ADSCs, known as the “Hayflick limit” in vitro, limits their clinical application. Here, we immortalized canine ADSCs (cADSCs) with SV40 gene and transplanted them into busulfan‐induced seminiferous tubules of infertile mice. The proliferation of these immortalized cells was improved significantly. Then, cellular differentiation assays showed that the immortalized cADSCs could differentiate into three‐germ‐layer cells, osteogenesis, chondrogenesis, adipogenesis phenotypes, and primordial germ cell‐like cells (PGCLCs). In addition, the immortalized cADSCs can proliferate in the busulfan‐induced seminiferous tubules of infertile mice. These findings confirmed that the immortalized cADSCs maintain the criteria of cADSCs.