Premium
Upregulation of PRMT6 by LPS suppresses Klotho expression through interaction with NF‐κB in glomerular mesangial cells
Author(s) -
Tsai KuenDaw,
Lee WenXi,
Chen Wei,
Chen BoYu,
Chen KuanLin,
Hsiao TzuChia,
Wang SueHong,
Lee YiJu,
Liang ShanYuan,
Shieh JiaChing,
Lin TingHui
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26511
Subject(s) - downregulation and upregulation , klotho , methylation , lipopolysaccharide , dna methylation , chemistry , epigenetics , immune system , microbiology and biotechnology , endocrinology , medicine , biology , gene expression , biochemistry , immunology , kidney , dna , gene
Lipopolysaccharide (LPS) released from gram‐negative bacteria stimulates immune responses in infected cells. Epigenetic modifications such as DNA methylation and protein methylation modulate LPS‐induced innate immune gene expressions. Expression of the Klotho protein decreased with LPS treatment in rats. In a cellular model, information regarding the effect of LPS on Klotho expression was meager. In the present study, we demonstrated that LPS triggered global DNA and protein methylation in glomerular mesangial MES‐13 cells. LPS upregulated protein expressions of enzymes central to cellular methylation reactions, especially protein arginine methyltransferase 6 (PRMT6) in MES‐13 cells. Expression of the Klotho protein was diminished by LPS and was restored by 5‐Aza‐2′‐deoxycytidine (5‐Aza‐2′‐dc), AMI‐1, and ammonium pyrrolidinedithiocarbamate (PDTC), but not adenosine aldehyde (AdOx). NF‐κB was identified as a substrate for arginine methylation and interacted with PRMT6 in MES‐13 cells. Inhibition of PRMT activity by AMI‐1 blocked LPS‐induced NF‐κB nuclear translocation in MES‐13 cells. Our data indicate that NF‐κB negatively regulated Klotho expression with an interaction with PRMT6, which was upregulated by LPS in MES‐13 cells.