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A CRISPR reimagining: New twists and turns of CRISPR beyond the genome‐engineering revolution
Author(s) -
Plummer Robert J.,
Guo Yi,
Peng Ying
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26406
Subject(s) - crispr , palindrome , genome editing , biology , computational biology , crispr interference , genome , chromatin , genome engineering , nuclease , dna , genetics , gene
Abstract Despite its explosive applications in genome engineering, CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) has been developed into a versatile tool beyond its well‐known nuclease function. In this prospect article, we summarize a few exciting “off‐label” applications of CRISPR including manipulating DNA sequences, visualizing chromosomal loci in living cells, and modulating transcription and chromatin structures. These novel applications will likely elevate CRISPR tools into yet another level of sophistication and diversity, leading to many more exciting cell biological discoveries.