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Regulation of fibroblast growth factor 8 ( FGF8 ) in chicken embryonic stem cells differentiation into spermatogonial stem cells
Author(s) -
Wang Man,
Zhang Chen,
Huang Chuanli,
Cheng Shaoze,
He Nana,
Wang Yilin,
Ahmed Mahmoud F.,
Zhao Ruifeng,
Jin Jing,
Zuo Qisheng,
Zhang Yani,
Li Bichun
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26402
Subject(s) - embryonic stem cell , stem cell , microbiology and biotechnology , fibroblast growth factor , biology , fgf8 , genetics , gene , receptor
Abstract Fibroblast growth factors (FGFs) are essential in regulating the formation of spermatogonial stem cells (SSCs). Here, we explored the effect of FGF8 on chicken SSCs formation by knockdown or overexpression of FGF8 in chicken embryonic stem cells (ESCs) both in vitro and in vivo. Our results showed that knockdown of FGF8 could facilitate the differentiation of ESCs into SSCs, overexpression of FGF8 could promote PGCs self‐renewal, inhibit SSCs formation. This study further revealed the positive correlation between the expression level of FGF8 and MAPK/ERK signal. In the absence of FGF8 , the expression of downstream genes such as FGFR2 , GRB2 , RAS , BRAF , RAF1 , and MEK2 was not maintained, while overexpressing FGF8 enhances them. Thus, our study demonstrated that FGF8 can regulate germ cell fate by modulating the dynamic equilibrium between differentiation and self‐renewal, which provides a new idea for the study of germ cell regulatory network.