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Silencing of SOCS‐1 and SOCS‐3 suppresses renal interstitial fibrosis by alleviating renal tubular damage in a rat model of hydronephrosis
Author(s) -
Zheng GuiHong,
Wang YongJian,
Wen Xin,
Han XinRui,
Shen Min,
Wang Shan,
Zhuang Juan,
Zhang ZiFeng,
Wang Liang,
Hu Bin,
Sun ChunHui,
Mao XiaoYun,
Yi LanNi,
Wu DongMei,
Lu Jun,
Zheng YuanLin
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26382
Subject(s) - gene silencing , hydronephrosis , kidney , cystatin c , small interfering rna , transforming growth factor , tumor necrosis factor alpha , creatinine , messenger rna , blot , biology , endocrinology , urinary system , transfection , biochemistry , gene
Our study was performed to elucidate how SOCS‐1/3 silencing suppresses renal interstitial fibrosis (RIF) by alleviating renal tubular damage in rat models affected by hydronephrosis. Male Wistar rats were randomly selected to establish hydronephrosis rat model, after which all rats were classified into normal, model, negative control (NC), siRNA‐SOCS‐1, siRNA‐SOCS‐3, and siRNA‐SOCS‐1 + siRNA‐SOCS‐3 groups. The levels of urine protein, serum creatinine (Scr), and blood urea nitrogen (BUN) were detected. ELISA was performed to determine levels of cystatin (CysC), β2‐microglobulin (β2‐MG), interleukin (IL)‐6, and tumor necrosis factor (TNF)‐α. RT‐qPCR and Western blotting were used for mRNA and protein expressions of SOCS‐1, SOCS‐3, α‐smooth muscle actin (α‐SMA), and transforming Growth Factor (TGF)‐β1. Compared with the normal group, the levels of Scr, BUN, urine protein, NAG, CysC, β2‐MG, IL‐6, and TNF‐α were increased in other groups, as well as elevated mRNA and protein expressions of SOCS‐1, SOCS‐3, α‐SMA, and TGF‐β1. The siRNA‐SOCS‐1, siRNA‐SOCS‐3, and siRNA‐SOCS‐1 + siRNA‐SOCS‐3 groups were found with decreased levels of Scr, BUN, urine protein, NAG, CysC, β2‐MG, IL‐6, and TNF‐α, as well as mRNA and protein expressions of SOCS‐1, SOCS‐3, α‐SMA, and TGF‐β1, including positive rates of SOCS‐1 and SOCS‐3 proteins in comparison with the model and NC groups. In comparison with the siRNA‐SOCS‐1 and siRNA‐SOCS‐3 groups, the siRNA‐SOCS‐1 + siRNA‐SOCS‐3 group exhibited decreased levels of Scr, BUN, urine protein, NAG, CysC, β2‐MG, IL‐6, and TNF‐α. Our study demonstrated that silencing of SOCS‐1/3 may suppress RIF by alleviating the renal tubular damage in rat models affected by hydronephrosis.