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MicroRNA‐622 suppresses the proliferation of glioma cells by targeting YAP1
Author(s) -
Xu Jin,
Ma Banyou,
Chen Gong,
Wei Dong,
Li Lixin,
Hu Weixing
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26343
Subject(s) - yap1 , glioma , microrna , cancer research , cell cycle , cell growth , function (biology) , luciferase , untranslated region , chemistry , cell , biology , microbiology and biotechnology , messenger rna , transfection , gene , biochemistry , transcription factor
It has recently been shown that miR‐622 plays a tumor suppressive role in many human cancers. However, the exact function and underlying mechanism are still unknown. Here, we reported that the level of miR‐622 is clearly reduced in human glioma tissues in comparison with normal brain tissues and is negatively correlated with the histological grades. Additionally, ectopically expressed miR‐622 significantly inhibited cell proliferation and induced cell cycle arrest at the G0/G1 phase in glioma cells. Furthermore, the bioinformatics analysis revealed that YAP1 possesses putative miR‐622—binding sites within its 3′UTR. Consequently, an elevated miR‐622 level was found to suppress the luciferase reporter activity of YAP1 3′UTR, and the effect was diminished by the deletion of the miR‐622 seed binding site. In addition, the level of YAP1 protein expression was significantly decreased after the overexpression of miR‐622. These results indicate a negative link between miR‐622 and YAP1 and further confirm that YAP1 is a direct target of miR‐622, suggesting that miR‐622 could be a new important therapeutic strategy for gliomas treatment.