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Retracted : Synergistic effect of siRNA‐mediated silencing of TGF‐β R1 and TGF‐β R2 genes on the proliferation and apoptosis of penile urethral epithelial cells in hypospadiac male rats
Author(s) -
Wang Chao,
Qiao YanYan
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26324
Subject(s) - gene silencing , transforming growth factor , apoptosis , small interfering rna , flow cytometry , chemistry , microbiology and biotechnology , cell growth , cell , messenger rna , cancer research , biology , transfection , gene , biochemistry
The study elucidated the effects associated with silencing growth factor‐β R1 ( TGF‐β R1 ) and TGF‐β R2 genes on the proliferation and apoptosis of penile urethral epithelial cells (UECs) in hypospadiac male rats. Seventy‐five male rats were distributed into the normal, model, TGF‐β R1/2 ‐siRNA, TGF‐β R1 ‐siRNA and TGF‐β R2 ‐siRNA groups. The UECs of the rats included in the study were cultured in vitro and subsequently divided into the control, blank, TGF‐β R1/2 ‐siRNA, TGF‐β R1 ‐siRNA and TGF‐β R2 ‐siRNA groups. The mRNA and protein expressions of TGF‐β R1 / R 2 were measured by quantitative real‐time polymerase chain reaction (qRT‐PCR) and western blotting. Cell proliferation and apoptosis were evaluated by cell counting kit‐8 (CCK‐8) assay as well as by flow cytometry. Compared with the normal group, the apoptotic rate of the UECs in the model, TGF‐β R1/2 ‐siRNA, TGF‐β R1 ‐siRNA and TGF‐β R2 ‐siRNA groups displayed remarkable increases; compared with the model group, the apoptotic rate of the UECs in the TGF‐β R1/2 ‐siRNA, TGF‐β R1 ‐siRNA and TGF‐β R2 ‐siRNA groups displayed significant decreases, similar observations were made regarding mRNA and protein expressions of TGF‐β R1 and TGF‐β R 2. Compared with the TGF‐β R1/2 ‐siRNA group, the apoptotic rates of the UECs in the TGF‐β R1 ‐siRNA and TGF‐β R2 ‐siRNA groups were up regulated, while cell proliferation in the TGF‐β R1 ‐siRNA and TGF‐β R2 ‐siRNA groups decreased accompanied by an increased rate of apoptosis. This study ultimately demonstrated that the silencing of TGF‐β R1 and TGF‐β R2 genes could promote cell proliferation and inhibit apoptosis of penile UECs in hypospadiac male rats.