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Induction of transient cell cycle arrest by H 2 O 2 via modulation of ultradian oscillations of Hes1, Socs3, and p ‐Stat3 in fibroblast cells
Author(s) -
Babaei Khalili Masoumeh,
Yazdanparast Razieh,
Nowrouzi Azin
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26306
Subject(s) - hes1 , microbiology and biotechnology , downregulation and upregulation , ultradian rhythm , oxidative stress , biology , degron , chemistry , signal transduction , endocrinology , ubiquitin ligase , biochemistry , circadian rhythm , notch signaling pathway , ubiquitin , gene
Biological clocks, time‐keeping systems, enable the living organisms to synchronize their biochemical processes with their environment. Among these molecular oscillators, ultradian oscillators have been identified with volatility less than 24 h. Transcription factor Hes1, a member of the basic Helix‐loop‐Helix (bHLH) protein family, has an oscillation duration of 2 h in vertebrates. Due to the pivotal role of oxidative stress in many human diseases, we evaluated the effect(s) of oxidative stress on Hes1 oscillator, its upstream regulators, and its downstream cell cycle regulators. NIH/3T3 mouse fibroblast cells were treated with sublethal (250 μM) and lethal (1000 μM) doses of H 2 O 2 for 30 min. H 2 O 2 generated a delay in p ‐Stat3 and Socs3 mRNAs followed by suppression of Hes1 protein. These events were accompanied by simultaneous upregulation of p21 and downregulation of cyclinD1, resulting in a temporary arrest of the cell cycle. In conclusion, the elimination of Hes1 protein oscillation by H 2 O 2 may represent a defense mechanism against oxidative stress in fibroblast cells.