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CREPT and p15RS regulate cell proliferation and cycling in chicken DF‐1 cells through the Wnt/β‐catenin pathway
Author(s) -
Jin Kai,
Chen Hao,
Zuo Qisheng,
Huang Chuanli,
Zhao Ruifeng,
Yu Xinjian,
Wang Yinjie,
Zhang Yani,
Chang Zhijie,
Li Bichu
Publication year - 2018
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26277
Subject(s) - wnt signaling pathway , cell growth , cell cycle , microbiology and biotechnology , cyclin d1 , gene knockdown , catenin , cell , small hairpin rna , biology , chemistry , signal transduction , cell culture , genetics
The CREPT (cell cycle‐related and expression elevated protein in tumor, also known as RPRD1B ) and p15RS (p15 INK4b ‐related sequence, also known as RPRD1A ) have been shown to regulate cell proliferation and alter the cell cycle through Wnt/β‐catenin pathway downstream genes in human. Although several studies have revealed the mechanism by which CREPT and p15RS regulate cell proliferation in human and mammals, it is still unclear how these genes function in poultry. In order to determine the function of CREPT and p15RS in chicken, we examined the expression of CREPT and p15RS in a variety of chicken tissues and DF‐1 cells. Then, we determined the effect of overexpression or depletion of CREPT or p15RS , by transiently transfecting chicken DF‐1 cells with overexpression and short hairpin RNA (shRNA) vectors respectively, on the regulation of cell proliferation. The results showed that CREPT and p15RS had different expression patterns and opposite effects on the cell cycling and proliferation. Knockdown of p15RS expression or overexpression of CREPT facilitated cell proliferation by promoting the cell‐cycle transition from G0/G1 to S‐phase and G2/M, whereas knockdown of CREPT or overexpression of p15RS inhibited cell proliferation. Mechanistically, CREPT and p15RS control DF‐1 cell proliferation by regulating the expression of Wnt/β‐catenin pathway downstream regulatory genes, including β‐catenin , TCF4 , and Cyclin D1 . In conclusion, CREPT and p15RS regulate cell proliferation and the cell‐cycle transition in chicken DF‐1 cells by regulating the transcription of Wnt/β‐catenin pathway downstream regulatory genes.