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Role of Small Interfering RNA Silencing Protein Kinase C‐α Gene on the Occurrence of Ultrafiltration Failure in Peritoneal Dialysis Rats
Author(s) -
Sun ZhiWei,
Wang Jian,
Weng Min,
Tang JianZhong,
Wang JunFeng,
Xu Jian,
Lin Ling,
Yuan HongLing
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26125
Subject(s) - uremia , peritoneal dialysis , gene silencing , protein kinase c , small interfering rna , western blot , gene expression , medicine , endocrinology , peritoneal cavity , andrology , microbiology and biotechnology , transfection , chemistry , phosphorylation , biology , gene , surgery , biochemistry
This study aims to explore the effect of PKC‐α gene silencing on the occurrence of ultrafiltration failure (UFF) in peritoneal dialysis (PD) rats. Forty‐eight male SD rats were collected to establish 5/6 renal resection uremic and uremic PD rats models. Rats were assigned into control, sham operation, uremia, PD‐2 W (peritoneal dialysis for 2 weeks), PD‐4 W (peritoneal dialysis for 4 weeks), negative control (NC) (peritoneal dialysis for 4 weeks, and injected 0.1 mg/kg blank plasmid into abdominal cavity) and PKC ‐ α siRNA (peritoneal dialysis for 4 weeks, and injected 0.1 mg/kg PKC ‐ α siRNA into abdominal cavity) groups. CD34 staining was performed to determine microvessel density (MVD) for peritoneal tissues. The mRNA and protein expression of PKC‐α in peritoneal tissue were detected by qRT ‐ PCR and Western blot. Compared with the control group, MVD, the mRNA and protein expression of PKC‐α were significantly increased in rats of the uremia, PD‐2 W, PD‐4 W, NC, and PKC ‐ α siRNA groups. Compared with the uremia group, MVD, the mRNA and protein expression of PKC ‐ α were increased, the changes observed in the PD‐4 W and NC groups were better obvious than in the PD‐2 W group. In comparison with the PD‐4 W and NC groups, MVD, the mRNA and protein expression of PKC ‐ α in rats were decreased in the PKC ‐ α siRNA group. PKC‐α gene has a high expression in uremic PD rats, and PKC‐α gene silencing is able to increase UF while decrease MVD and glucose transport in peritoneal tissues thus reversing UFF in PD rats. J. Cell. Biochem. 118: 4607–4616, 2017. © 2017 Wiley Periodicals, Inc.