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Measuring Kinase Activity—A Global Challenge
Author(s) -
Cann Marissa L.,
McDonald Ian M.,
East Michael P.,
Johnson Gary L.,
Graves Lee M.
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26103
Subject(s) - kinome , kinase , motility , biology , signal transduction , microbiology and biotechnology , protein kinase a , ask1 , computational biology , cyclin dependent kinase 2
The kinase enzymes within a cell, known collectively as the kinome, play crucial roles in many signaling pathways, including survival, motility, differentiation, stress response, and many more. Aberrant signaling through kinase pathways is often linked to cancer, among other diseases. A major area of scientific research involves understanding the relationships between kinases, their targets, and how the kinome adapts to perturbations of the cellular system. This review will discuss many of the current and developing methods for studying kinase activity, and evaluate their applications, advantages, and disadvantages. J. Cell. Biochem. 118: 3595–3606, 2017. © 2017 Wiley Periodicals, Inc.