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Regulation of Histone Acetylation on Expression Profiles of Potassium Channels During Cardiomyocyte Differentiation From Mouse Embryonic Stem Cells
Author(s) -
Wang Duo,
Liu Chang,
Li Zhigang,
Wang Yumei,
Wang Wenjing,
Wu Xiujuan,
Wang Kang,
Miao Wei,
Li Li,
Peng Luying
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26102
Subject(s) - potassium channel , inward rectifier potassium ion channel , microbiology and biotechnology , acetylation , histone , embryonic stem cell , chemistry , cellular differentiation , biology , ion channel , biochemistry , biophysics , receptor , gene
The cardiomyocyte differentiation from mouse embryonic stem cells (mESCs) is a dynamic and complex process that involved in the precision regulation of histone acetylation. The formation of action potential (AP) in mature cardiomyocytes is based on the expression pattern of Na + , Ca 2+ , and K + ion channels, in which the slow delayed rectifier potassium current (I Ks ), the rapid delayed rectifier potassium current (I Kr ) and the inwardly rectifying Kir current (I K1 ) mainly contribute to repolarization for AP in different species. However, the expression status of potassium channels conducted I Ks , I Kr , and I K1 in cardiomyocyte differentiation are not fully defined. Here, we investigated the expression pattern of the slow delayed rectifier potassium channel and the rapid delayed rectifier potassium channel using a model of mouse cardiomyocyte differentiation under different conditions of histone acetylation. We found that expression levels of both the delayed rectifier potassium channel and the inwardly rectifying potassium channel were more sensitive to histone hyperacetylation during differentiation from mESCs into cardiomyocytes. Especially, histone H4 hyperacetylation induced by Class I HDACs inhibitors promoted the expression profiles of potassium channels (Kcnj2, Kcnj3, Kcnj5, Kcnj11, and Kcnh2) in the process. Our results provide a clue for expression status of potassium channels which may be essential to forming functional cardiomyocyte in the cardiac lineage commitment of mESC. J. Cell. Biochem. 118: 4460–4467, 2017. © 2017 Wiley Periodicals, Inc.