High Dose Supplementation of Vitamin D Affects Measures of Systemic Inflammation: Reductions in High Sensitivity C‐Reactive Protein Level and Neutrophil to Lymphocyte Ratio (NLR) Distribution

The prevalence of Vitamin D deficiency is increasing worldwide, which has be shown to be associated with increased risk of cardiovascular disease (CVD), autoimmune disease, and metabolic syndrome. These conditions are also associated with a heightened state of inflammation. The aim of the current study was to evaluate the effect of vitamin D supplementation on serum C‐Reactive Protein (CRP) level and Neutrophil‐to‐lymphocyte ratio (NLR) distribution in a large cohort of adolescent girls. A total of 580 adolescent girls were recruited follow by evaluation of CRP and hematological parameters before and after supplementation with vitamin D supplements as 9 of 50000 IU cholecalciferol capsules for 9 weeks taken at weekly intervals. At baseline, serum hs‐CRP level was 0.9 (95%CI: 0.5–1.8), while this value after intervention was reduced to 0.8 (95%CI: 0.3–1.6; P  = 0.007). Similar results were also detected for NLR (e.g., NLR level was 1.66 ± 0.72 and 1.53 ± 0.67, P  = 0.002, before and after therapy with compliance rate of >95.2%). Moreover, we found an association between hs‐CRP and BMI, triglyceride, white blood cell count, and lymphocytes. Interestingly we observed a significant reduction in neutrophil count and CRP level after high dose vitamin D supplementation. Our findings showed that the high dose supplementation of vitamin D affects measures of systemic inflammation: reductions in High Sensitivity C‐Reactive Protein level and Neutrophil‐to‐lymphocyte ratio (NLR) distribution. J. Cell. Biochem. 118: 4317–4322, 2017. © 2017 Wiley Periodicals, Inc.