Premium
Exosomes Transfer Among Different Species Cells and Mediating miRNAs Delivery
Author(s) -
Zhou Yueyuan,
Tian Tian,
Zhu Yanliang,
Jaffar Ali Doulathunnisa,
Hu Feihu,
Qi Yuhua,
Sun Bo,
Xiao Zhongdang
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26077
Subject(s) - microvesicles , exosome , microrna , internalization , microbiology and biotechnology , biology , hela , cell , gene , genetics
Exosomes, the natural vehicles of intercellular communication, transfer proteins, mRNAs, and microRNAs (miRNAs) and mediate many physiological and pathological processes. It is not clear that whether exosomal miRNAs could regulate gene expression across species, though some studies suggest interactions of exosomal miRNAs between cells. In this report, we have isolated exosomes from rat PC12 cells and assessed their internalization by human cancer Hela cells. The internalized exosomes were located in Hela lysosomes. Human PTEN expression was significantly deregulated due to miR‐21 delivered by rat cell exosomes. Our results prove that exosomes could incorporate between cells of different species and could regulate the protein expressions in the recipient cells by delivering the enclosed miRNAs. Thus our study foreshadows a futuristic treatment approach of utilizing miRNA enclosed exosome vehicles sans species concerns in combating various diseases/ regulating abnormal proteins. J. Cell. Biochem. 118: 4267–4274, 2017. © 2017 Wiley Periodicals, Inc.