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Flavonoids of Rosa roxburghii Tratt Exhibit Anti‐Apoptosis Properties by Regulating PARP‐1/AIF
Author(s) -
Xu Ping,
Liu Xingxia,
Xiong Xiwen,
Zhang Wenbo,
Cai Xinhua,
Qiu Peiyong,
Hao Minghua,
Wang Lijuan,
Lu Dandan,
Zhang Xiuhua,
Yang Wancai
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26049
Subject(s) - p38 mitogen activated protein kinases , apoptosis , poly adp ribose polymerase , apoptosis inducing factor , mapk/erk pathway , microbiology and biotechnology , chemistry , mitochondrion , signal transduction , cytoplasm , programmed cell death , cancer research , biology , caspase , biochemistry , dna , polymerase
ABSTRACT Radioprotection is an important approach to reduce the side‐effects of radiotherapy. The radioprotective effect of the flavonoids of Rosa roxburghii Tratt (FRT) has been confirmed, and the mechanism has been identified as theBcl‐2/caspase‐3/PARP‐1 signaling pathway. In this study, we investigated the effects of FRT on the intercellular adhesion molecule (ICAM), and vascular cell adhesion protein (VCAM) in addition to apoptosis‐related proteins such as Bax/Bcl‐2, p‐ERK/ERK, p‐p53/p53, and p‐p38/p38. In the present study, we focused on the effect of FRT on PARP‐1/AIF. Ionizing radiation triggered the activation of PARP‐1 and AIF translocation from the mitochondrion to the nucleus. The inhibition of PARP‐1/AIF signaling pathway by FRT was investigated. Our results showed that the expressions of Bax/Bcl‐2, p‐ERK/ ERK, p‐p53/p53, and p‐p38/p38 were decreased after FRT treatment compared with the radiation‐treated group. FRT inhibited PARP‐1 activation to inhibit AIF translocation from mitochondrion to nucleus. Pretreatment with FRT diminished the comet's tail and reduced fragments in six Gy‐irradiated thymocytes compared with the irradiated cells without FRT treatment. We conclude that FRT enhanced radioprotection at least partially by regulating PARP‐1/AIF to reduce apoptosis. J. Cell. Biochem. 118: 3943–3952, 2017. © 2017 Wiley Periodicals, Inc.