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Retracted : MicroRNA‐126 Inhibits Proliferation, Migration, Invasion, and EMT in Osteosarcoma by Targeting ZEB1
Author(s) -
Jiang Rui,
Zhang Chao,
Liu Guangyao,
Gu Rui,
Wu Han
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26024
Subject(s) - osteosarcoma , microrna , cancer research , biology , microbiology and biotechnology , gene , genetics
Osteosarcoma (OS) is a tumor with rapid progression, high metastatic potential and poor clinical prognosis. This study was aimed to investigate the function of miR‐126 in OS cells. The miR‐126 expression in OS cell lines and OS tissues were explored by qRT‐PCR. Then, the effects of miR‐126 on proliferation, cycle, migration, invasion, and transforming growth factor (TGF)‐β1 induced epithelial to mesenchymal transition (EMT) were assessed. Predicted by TargetScan, one of target genes for miR‐126 was verified by luciferase activity assay. Meantime, the mRNA and protein expressions of ZEB1 were assessed by qRT‐PCR and Western blot assay. Subsequently, the effect of ZEB1 silence on miR‐126 down‐regulated cells was also evaluated. Finally, the expressions of key kinases involved in c‐Jun N‐terminal kinase (JNK) and Janus‐activated kinase (JAK)‐1/signal transducer and activator of transcription (STAT)‐3 pathways were detected by Western blot analysis. Result showed that miR‐126 was down‐regulated in OS tissues and cell lines. Overexpression of miR‐126 significantly inhibited cell proliferation, migration, invasion, and TGF‐β1 induced EMT. The effect of miR‐126 knockdown was just the opposite. ZEB1 was predicted and verified as a target gene of miR‐126. Meantime, the influence of miR‐126 knockdown was abrogated by ZEB1 silence. Additionally, the phosphorylation levels of c‐Jun, JNK, JAK1, and STAT3 were down‐regulated in miR‐126 over‐expressed cells, and the effect of miR‐126 knockdown was reversed by ZEB1 silence. In conclusion, miR‐126 inhibits proliferation, migration, invasion and EMT in OS by targeting ZEB1 through inactivation of JNK and JAK1/STAT3 pathways. J. Cell. Biochem. 118: 3765–3774, 2017. © 2017 Wiley Periodicals, Inc.