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MiR‐223/PAX6 Axis Regulates Glioblastoma Stem Cell Proliferation and the Chemo Resistance to TMZ via Regulating PI3K/Akt Pathway
Author(s) -
Huang BaiSheng,
Luo Qizhi,
Han Yang,
Huang Dong,
Tang QingPing,
Wu LiXiang
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.26003
Subject(s) - temozolomide , pi3k/akt/mtor pathway , glioma , protein kinase b , cancer research , pax6 , signal transduction , cell growth , biology , chemistry , microbiology and biotechnology , transcription factor , genetics , gene
Chemotherapy is a standard strategy for glioma, while chemoresistance remains a major therapeutic challenge in current clinical practice. Our present study was aimed to determine whether inhibition of the miR‐223/paired box 6 (PAX6) pathway could increase the sensitivity of glioma to Temozolomide. An elevated level of miR‐223 was observed in glioma tissues. Exogenous miR‐223 promoted cell survival when exposed to Temozolomide (TMZ), while miR‐223 inhibition could reverse this process. The RNA and protein levels of PAX6 were significantly decreased by exogenous miR‐223, and the 3′‐untranslated region of PAX6 was shown to be a target of miR‐223. Besides, it has also been reported that PI3K/Akt signaling pathway is pivotal to regulate glioma growth and proliferation. In the present study, we revealed that miR‐223/PAX6 axis regulated the growth, invasion, and chemo resistance of glioblastoma stem cells to TMZ via regulating PI3K/Akt signaling pathway, which present a novel potential therapy for intervention of glioblastoma. Taken together, our findings shed new light on the miR‐223/PAX6 pathway in glioma and this pathway might modulate the sensitivity of glioma to TMZ via regulating PI3K/Akt signaling pathway. J. Cell. Biochem. 118: 3452–3461, 2017. © 2017 Wiley Periodicals, Inc.